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mouse eda a1  (OriGene)


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    OriGene mouse eda a1
    Mouse Eda A1, supplied by OriGene, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+eda+a1/Eda+(NM_010099)+Mouse+Untagged+Clone/pm37165186-366-0-18
    Average 91 stars, based on 2 article reviews
    mouse eda a1 - by Bioz Stars, 2026-09
    91/100 stars

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    Related Articles

    Expressing:

    Article Title: EDA2R-NIK signalling promotes muscle atrophy linked to cancer cachexia.
    Article Snippet: Skeletal muscle atrophy is a hallmark of the cachexia syndrome that is associated with poor survival and reduced quality of life in patients with cancer.. Muscle atrophy involves excessive protein catabolism and loss of muscle mass and strength.. An effective therapy against muscle wasting is currently lacking because mechanisms driving the atrophy process remain incompletely understood.

    Article Title: EDA2R/NIK signaling promotes skeletal muscle atrophy linked to cancer cachexia
    Article Snippet: Small molecule inhibitors were acquired from indicated sources: TPCA-1 (abcam, ab145522), MG132 (Sigma; M8699), BAY 11-7082 (abcam, ab141228), BOT-64 (Santa Cruz; sc-222062), B022 (Aobious; AOB8699). .. Mouse EDA-A1 (MC208411), mouse EDA-A2 (MC208415) and mouse OSM (MR226014) expression plasmids were purchased from Origene. .. Mouse NIK expression plasmid was purchased from Invivogen (pUNO1-mMap3k14).



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    Corneal epithelial wound healing delayed in Tabby mice and promoted by exogenous Eda. A, 2.0-mm central corneal epithelial debridement was performed in 8-week-old wild-type mice and Tabby mice. Sodium fluorescein staining showed gradual wound closure. The corneal epithelium was almost completely healed in wild-type mice at 24 h after wounding, whereas the epithelial defect was not completely resurfaced even at 36 h after the wounding in Tabby mice. B, 2.5-mm central corneal epithelial debridement wound was created in 6-week-old Tabby mice, 100 ng/ml of mouse <t>recombinant</t> Eda protein in PBS was applied in the conjunctival sac of Tabby mice, four times per day. PBS alone was used as control. Sodium fluorescein staining showed that the epithelial wound was not healed at 36 h in the PBS group, whereas was almost completely healed in the Eda-treated group. C, for the epithelial wound healing in Tabby mice and wild-type mice, the extent of the wound closure rate at 12 h was 48.9% in Tabby mice and 59.1% in the wild-type mice. It was 67.5% in the Tabby mice and 99.3% in the wild-type mice at 24 h. Tabby mice showed significant delayed corneal wound healing at both time points (n = 4, *, p < 0.05; ***, p < 0.001). D, for the epithelial wound healing in Tabby mice with or without Eda treatment, extent of the wound closure rate at 12 h was 52.5% in the Eda-treated group (Ta-Eda) and 34.3% in Tabby mice treated with PBS alone (Ta-PBS). It was 65.1% in Ta-Eda and 50.4% in Ta-PBS at 24 h, 92.8% in Ta-Eda and 80.6% in Ta-PBS at 36 h. Eda treatment significantly promoted wound healing in Tabby mice at all the time points (n = 4, *, p < 0.05).
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    Corneal epithelial wound healing delayed in Tabby mice and promoted by exogenous Eda. A, 2.0-mm central corneal epithelial debridement was performed in 8-week-old wild-type mice and Tabby mice. Sodium fluorescein staining showed gradual wound closure. The corneal epithelium was almost completely healed in wild-type mice at 24 h after wounding, whereas the epithelial defect was not completely resurfaced even at 36 h after the wounding in Tabby mice. B, 2.5-mm central corneal epithelial debridement wound was created in 6-week-old Tabby mice, 100 ng/ml of mouse recombinant Eda protein in PBS was applied in the conjunctival sac of Tabby mice, four times per day. PBS alone was used as control. Sodium fluorescein staining showed that the epithelial wound was not healed at 36 h in the PBS group, whereas was almost completely healed in the Eda-treated group. C, for the epithelial wound healing in Tabby mice and wild-type mice, the extent of the wound closure rate at 12 h was 48.9% in Tabby mice and 59.1% in the wild-type mice. It was 67.5% in the Tabby mice and 99.3% in the wild-type mice at 24 h. Tabby mice showed significant delayed corneal wound healing at both time points (n = 4, *, p < 0.05; ***, p < 0.001). D, for the epithelial wound healing in Tabby mice with or without Eda treatment, extent of the wound closure rate at 12 h was 52.5% in the Eda-treated group (Ta-Eda) and 34.3% in Tabby mice treated with PBS alone (Ta-PBS). It was 65.1% in Ta-Eda and 50.4% in Ta-PBS at 24 h, 92.8% in Ta-Eda and 80.6% in Ta-PBS at 36 h. Eda treatment significantly promoted wound healing in Tabby mice at all the time points (n = 4, *, p < 0.05).

    Journal: The Journal of Biological Chemistry

    Article Title: Ectodysplasin A protein promotes corneal epithelial cell proliferation

    doi: 10.1074/jbc.M117.803809

    Figure Lengend Snippet: Corneal epithelial wound healing delayed in Tabby mice and promoted by exogenous Eda. A, 2.0-mm central corneal epithelial debridement was performed in 8-week-old wild-type mice and Tabby mice. Sodium fluorescein staining showed gradual wound closure. The corneal epithelium was almost completely healed in wild-type mice at 24 h after wounding, whereas the epithelial defect was not completely resurfaced even at 36 h after the wounding in Tabby mice. B, 2.5-mm central corneal epithelial debridement wound was created in 6-week-old Tabby mice, 100 ng/ml of mouse recombinant Eda protein in PBS was applied in the conjunctival sac of Tabby mice, four times per day. PBS alone was used as control. Sodium fluorescein staining showed that the epithelial wound was not healed at 36 h in the PBS group, whereas was almost completely healed in the Eda-treated group. C, for the epithelial wound healing in Tabby mice and wild-type mice, the extent of the wound closure rate at 12 h was 48.9% in Tabby mice and 59.1% in the wild-type mice. It was 67.5% in the Tabby mice and 99.3% in the wild-type mice at 24 h. Tabby mice showed significant delayed corneal wound healing at both time points (n = 4, *, p < 0.05; ***, p < 0.001). D, for the epithelial wound healing in Tabby mice with or without Eda treatment, extent of the wound closure rate at 12 h was 52.5% in the Eda-treated group (Ta-Eda) and 34.3% in Tabby mice treated with PBS alone (Ta-PBS). It was 65.1% in Ta-Eda and 50.4% in Ta-PBS at 24 h, 92.8% in Ta-Eda and 80.6% in Ta-PBS at 36 h. Eda treatment significantly promoted wound healing in Tabby mice at all the time points (n = 4, *, p < 0.05).

    Article Snippet: A 2.5-mm diameter corneal epithelial debridement wound was created on the central corneas of 6-week-old Tabby mice, 5 μl of 100 ng/ml of mouse recombinant Eda protein (191-ED, R&D Systems) in PBS was applied in their conjunctival sac four times per day.

    Techniques: Staining, Recombinant, Control

    Eda promotes corneal epithelial wound healing during in vitro organ culture. A, 8-week-old wild-type mice eyeballs with a 2.0-mm corneal epithelial debridement wound on the central cornea were cultured in DMEM + 2% FBS in the absence or presence of 20 ng/ml of recombinant Eda protein or 10 ng/ml of EGF protein for different durations from 12 to 36 h. Fluorescein staining evaluated differences in wound closure extent during the culture. B, the wound closure extent reached 26.5% in the Eda treatment group (Eda), 28.7% in the EGF treatment group (EGF), whereas it was 9.6% in the control group (NC) after 12 h culture. At 24 h, the wounds had healed to 80.0% in the Eda treatment group, whereas it was 58.7% in the control group, and completed healed in the EGF group. At 36 h, the epithelial defect was completely healed in both groups (n = 4, *, p < 0.05). C, after a 36-h culture, H&E staining of the eyeballs showed there was a single layer of epithelial cells present in the central cornea of the control eyeballs, whereas there were two to three layers of epithelial cells in the EGF or Eda-treated corneas. Scale bar represents 50 μm.

    Journal: The Journal of Biological Chemistry

    Article Title: Ectodysplasin A protein promotes corneal epithelial cell proliferation

    doi: 10.1074/jbc.M117.803809

    Figure Lengend Snippet: Eda promotes corneal epithelial wound healing during in vitro organ culture. A, 8-week-old wild-type mice eyeballs with a 2.0-mm corneal epithelial debridement wound on the central cornea were cultured in DMEM + 2% FBS in the absence or presence of 20 ng/ml of recombinant Eda protein or 10 ng/ml of EGF protein for different durations from 12 to 36 h. Fluorescein staining evaluated differences in wound closure extent during the culture. B, the wound closure extent reached 26.5% in the Eda treatment group (Eda), 28.7% in the EGF treatment group (EGF), whereas it was 9.6% in the control group (NC) after 12 h culture. At 24 h, the wounds had healed to 80.0% in the Eda treatment group, whereas it was 58.7% in the control group, and completed healed in the EGF group. At 36 h, the epithelial defect was completely healed in both groups (n = 4, *, p < 0.05). C, after a 36-h culture, H&E staining of the eyeballs showed there was a single layer of epithelial cells present in the central cornea of the control eyeballs, whereas there were two to three layers of epithelial cells in the EGF or Eda-treated corneas. Scale bar represents 50 μm.

    Article Snippet: A 2.5-mm diameter corneal epithelial debridement wound was created on the central corneas of 6-week-old Tabby mice, 5 μl of 100 ng/ml of mouse recombinant Eda protein (191-ED, R&D Systems) in PBS was applied in their conjunctival sac four times per day.

    Techniques: In Vitro, Organ Culture, Cell Culture, Recombinant, Staining, Control